Skip Ribbon Commands
Skip to main content

Equipment Detail

Name

MiSeq Desktop Sequencer

Manufacturer

Illumina

Make or Model

n/a

Description

Focused Power:

Speed and simplicity for targeted and small genome sequencing.

​The MiSeq desktop sequencer allows you to access more focused applications such as targeted gene sequencing, metagenomics, small genome sequencing, targeted gene expression, amplicon sequencing, and HLA typing. New MiSeq reagents enable up to 15 Gb of output with 25 M sequencing reads and 2x300 bp read lengths.

MiSeq Specifications

 
 

Cluster Generation and Sequencing

MiSeq Reagent Kit v2
Read Length Total Time* Output
1 × 36 bp
~4 hrs 540-610 Mb
2 × 25 bp
~5.5 hrs 750-850 Mb
2 × 150 bp
~24 hrs 4.5-5.1 Gb
2 × 250 bp ~39 hrs 7.5-8.5 Gb
MiSeq Reagent Kit v3
Read Length Total Time* Output
2 × 75 bp ~20 hrs
3.3-3.8 Gb
2 × 300 bp ~55 hrs
13.2-15 Gb
 
 

Reads Passing Filter†

MiSeq Reagent Kit v2
Single Reads 12-15 M
Paired-End Reads 24-30 M
MiSeq Reagent Kit v3
Single Reads 22-25 M
Paired-End Reads 44-50 M
 
 

Quality Scores††

MiSeq Reagent Kit v2
> 90% bases higher than Q30 at 1 x 36 bp
> 90% bases higher than Q30 at 2 x 25 bp
> 80% bases higher than Q30 at 2 x 150 bp
> 75% bases higher than Q30 at 2 x 250 bp
MiSeq Reagent Kit v3
> 85% bases higher than Q30 at 2 x 75 bp
> 70% bases higher than Q30 at 2 x 300 bp
 
*Total time includes cluster generation, sequencing, and basecalling on a MiSeq System enabled with dual-surface scanning.
†Install specifications based on Illumina PhiX control library at supported cluster densities (between 467-583 k/mm2 clusters passing filter for v2 chemistry and 727-827 k/mm2 clusters passing filter for v3 chemistry). Actual performance parameters may vary based on sample type, sample quality, and clusters passing filter.
††A quality score (Q-score) is a prediction of the probability of an error in base calling. The percentage of bases > Q30 is averaged across the entire run.
bp = base pairs, Mb = megabases, Gb = gigabases, M = millions.


 

 





 
 


External Link

http://www.illumina.com/systems/miseq.html

Picture

Contact Information

Contact Name

Jose V. Lopez, Ph.D.

Telephone Number

954 262 3665

Fax

954 262 2658

Email

joslo@nova.edu
lssf footer